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| Registros recuperados: 136 | |
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| Taris, Nicolas; Baron, S; Sharbel, Tim; Sauvage, Christopher; Boudry, Pierre. |
| The analysis of parentage using microsatellite markers is of increasing importance, notably in aquaculture genetic research where communal rearing of mixed families can be used to reduce unwanted environmental variance. We present here an optimization of parental genotype assessment for larvae or adults of the Pacific oyster, Crassostrea gigas, using a multiplex system of three microsatellite loci. In conjunction with a simple DNA extraction protocol, this method enables high throughput analyses of parentage in C. gigas. Using this method, we successfully determined the parentage of 93% (1224 out of 1318) of the progeny in a factorial cross between 3 females and 10 males. The inability to genotype the remaining 7% was due to DNA degradation of larvae... |
| Tipo: Text |
Palavras-chave: Oysters; Aquaculture; Parental assignment; High throughput; Larval DNA extraction; Multiplex. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-1814.pdf |
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| Arzul, Isabelle; Miossec, Laurence; Blanchet, Estelle; Garcia, Celine; Joly, Jean-pierre; Francois, Cyrille; Berthe, Franck. |
| Bonamiosis was first reported in association with mass mortality of flat oysters, Ostrea edulis, in June 1979 in L'Ile Tudy, Brittany. The disease rapidly spread to all of the oyster farming areas in France but also in other European countries. The French flat oyster production, which already suffered from another protozoan disease, marteiliosis, decreased from 5500 mt in 1979 to less than 2000 mt after 1980. In 2001, about 350 French farms sell 1650 mt of flat oysters. This production mainly relies on natural spat collection which specially occurs in Quiberon bay. One third of this spat is transferred from South to North Brittany for further growth. Quiberon bay constitutes an interesting site regarding the surveillance of bonamiosis because of the... |
| Tipo: Text |
Palavras-chave: Detection; Bonamia ostreae; Protozoan; Diseases; Pathology; Ostrea edulis; Oysters. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/acte-3314.pdf |
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| Bacher, Cedric; Baud, Jean-pierre; Bodoy, Alain; Deslous-paoli, Jean-marc; Dreno, Jean-paul; Heral, Maurice; Maurer, Daniele; Prou, Jean. |
| An assessment of the reared stocks of the Pacific oyster (Crassostrea gigas ) has been recently conducted on the major French areas of molluscs culture. The methodology proposed included both estimates of densities obtained by subsampling in the field, and aerial photographs which covered the cultivated, intertidal area. The size and the number of samples were designed to reach a precision of 10% on the final assessment in each area. The field work consisted in weighing and counting the oyster in unit areas of 0.5 m2 for the bottom culture or in trays or bags, for the off bottom technique. These results give a better knowledge of the technique evolution and of the general structures in the cultivated areas. |
| Tipo: Text |
Palavras-chave: Modeling; Estimation; Stocks; Crassostrea gigas; Oysters. |
| Ano: 1986 |
URL: http://archimer.ifremer.fr/doc/1986/acte-2864.pdf |
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| Ludwig,Sandra; Patella,Raquel; Stoiev,Sérgio; Castilho-Westphal,Gisela; Girotto,Marcus V. F; Ostrensky,Antonio. |
| Among oysters, species of Crassostrea (Sacco, 1897) are the most attractive to aquaculture. In Brazil, the genus is represented by C. rhizophorae (Guilding, 1828) and C. brasiliana (Lamarck, 1819). Because the maturation and breeding technology is not well developed for these species, aquaculturists need a reliable method to decide the correct time to place spat collectors in the field, and to identify both species, which are morphologically similar. In this study a specific Multiplex PCR protocol was developed, using one pair of universal primers from 18S rDNA as a positive control and a pair of specific primers for each target species. The sensitivity and specificity of the protocol was evaluated. It detected C. rhizophorae DNA in low concentrations, and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Multiplex PCR; Oysters; Plankton; Specific primers. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702011000400002 |
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| Hartnell, R. E.; Stockley, L.; Keay, W.; Rosec, J. -p.; Hervio-heath, Dominique; Van Den Berg, H.; Leoni, F.; Ottaviani, D.; Henigman, U.; Denayer, S.; Serbruyns, B.; Georgsson, F.; Krumova-valcheva, G.; Gyurova, E.; Blanco, C.; Copin, S.; Strauch, E.; Wieczorek, K.; Lopatek, M.; Britova, A.; Hardouin, G.; Lombard, B.; Veld, P. In'T; Leclercq, A.; Baker-austin, C.. |
| Globally, vibrios represent an important and well-established group of bacterial foodborne pathogens. The European Commission (EC) mandated the Comite de European Normalisation (CEN) to undertake work to provide validation data for 15 methods in microbiology to support EC legislation. As part of this mandated work programme, merging of ISO/TS 21872–1:2007, which specifies a horizontal method for the detection of V. parahaemolyticus and V. cholerae, and ISO/TS 21872–2:2007, a similar horizontal method for the detection of potentially pathogenic vibrios other than V. cholerae and V. parahaemolyticus) was proposed. Both parts of ISO/TS 21872 utilized classical culture-based isolation techniques coupled with biochemical confirmation steps. The work also... |
| Tipo: Text |
Palavras-chave: Vibrios; Seafood; Prawns; Oysters; Biochemical methods; Real-time PCR; Conventional PCR. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00425/53680/54525.pdf |
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| Huvet, Arnaud; Samain, Jean-francois; Boudry, Pierre; Bedier, Edouard; Ropert, Michel; Van Wormhoudt, A.. |
| The better understanding of physiological and environmental factors that determine optimal food conversion efficiencies is of major interest for the cupped oyster Crassostrea gigas for which the strong increase of aquaculture has been correlated in France with a decrease in productivity due to competition between aquatic species for limited food supplies at grow-out sites. To investigate the non-neutrality of the polymorphism of amylase, a key enzyme for carbohydrate assimilation, in oyster physiology, we report in the present paper the growth and survival measurement of amylase genotypes within five bi-parental C. gigas families, bred to be polymorph at the two PCR-RFLP amylase markers, and reared over one year under standard culture conditions in two... |
| Tipo: Text |
Palavras-chave: Amylase markers; Growth; Amylase Polymorphism; Genetic; Oysters; Crassostrea gigas. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/acte-3311.pdf |
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| Lapegue, Sylvie; Bedier, Edouard; Goyard, Emmanuel; Degremont, Lionel; Baud, Jean-pierre; Gerard, Andre; Goulletquer, Philippe; Boudry, Pierre. |
| Two oyster species are currently present along the French coasts : the indigenous European flat oyster (Ostrea edulis), and the Pacific cupped oyster (Crassostrea gigas), that has been introduced from Japan since the beginning of the 70ies. The flat oyster successively suffered from two protozoan diseases during the 60ies and its production decreased from 20 000 tons/year by that time to 1 500 tons/year nowadays. Consequently, the oyster production is principally (99%) based upon the Pacific oyster species with approximately 150 000 tons/year among which 90% are grown from the natural spat. However, the hatchery production of this species is developing and was estimated to 400 to 800 millions spat in 2002. Moreover, strengthened relationships between... |
| Tipo: Text |
Palavras-chave: Growth; Selection; Genetic breeding; Genetic; Ostrea edulis; Crassostrea gigas; Oysters; Croissance; Sélection; Amélioration génétique; Génétique; Ostrea edulis; Crassostrea gigas; Huîtres. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2003/acte-3491.pdf |
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| Bodoy, Alain. |
| The natural mortality of oysters could by considered, in a man controlled ecosystem, as the result of the action of biological factors, and human factors other then the harvest of the population. Such mortalities need to be estimated in order to be used in population dynamics, as a tool for management purposes. ln Marennes-Oleron bay, different ways of estimating the natural mortality are available. Several oyster farms were monitored for assessing growth, mortality and production in rearing conditions. Furthermore, an experimental framework which includes 15 stations gave an appraisal of the same factors under controlled conditions. Finally, another estimate will be soon available by comparin9 the number of recruited oysters, and the number of oyster... |
| Tipo: Text |
Palavras-chave: Marennes Oleron; Mortality; Crassostrea gigas; Oysters. |
| Ano: 1986 |
URL: http://archimer.ifremer.fr/doc/1986/acte-2962.pdf |
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| Li, Meng; Callier, Myriam; Blancheton, Jean-paul; Galès, Amandine; Nahon, Sarah; Triplet, Sebastien; Geoffroy, Thibault; Menniti, Christophe; Fouilland, Eric; Roque D'Orbcastel, Emmanuelle. |
| Integrated multi-trophic aquaculture (IMTA) systems are a promising solution for sustainable aquaculture combining nutrient recycling with increased biomass production. An innovative land-based recirculating aquaculture system (RAS) was studied in France for a 60-day experiment. It combined a European sea bass (Dicentrarchus labrax) RAS with two other production systems: high rate algal ponds (HRAP) with natural marine polyspecific algal assemblages, and oysters in separate open tanks. The objective was the assessment of: 1) the efficiency and the stability of the microalgae bioremediation of the effluent from a fish RAS in spring and summer, 2) the abundance and the diversity patterns of the microalgae biomass for consumption in the oyster compartment of... |
| Tipo: Text |
Palavras-chave: Microalgae; IMTA; Nutrient bioremediation; Community structure; Oysters. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00480/59207/61900.pdf |
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| Grizel, Henri; Mialhe, Eric; Chagot, Dominique; Boulo, Viviane; Bachere, Evelyne. |
| Research on bonamiasis, an epizootic disease of the edible oyster Ostrea edu/is caused by the protozoan Bonamia ostreae, is discussed in relation to oyster fanning, research technology, epizootiology, and management. Morphological and infectious characteristics of the parasite are described. Recent progress in isolation and purification of the parasite have permitted investigations into host defense mechanisms, parasite infectivity, and the development of a mol1usc-pathogen mode!. |
| Tipo: Text |
Palavras-chave: Epizootic disease; Pathology; Protozoan; Bonamia ostreae; Oysters. |
| Ano: 1988 |
URL: http://archimer.ifremer.fr/doc/1988/publication-3120.pdf |
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| Registros recuperados: 136 | |
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